Mutational_Signatures_in_the_YKO_collection. Mutational_Signatures_in_the_YKO_collection

This project is being carried out through a Strategic Award from the Wellcome Trust to Steve Jackson, Mike Stratton, Peter Campbell, Serena Nik-Zainal and others. This grant is to fund work aimed at identifying “mutational signatures” arising through cells being defective in DNA repair and other cellular factors, and/or through cellular exposure to various DNA damaging agents. The samples that are here being provided here are derived from the yeast Saccharomyces cerevisiae, and represent wild-type strains plus various gene knock-out strains that have or have not been propagated over several months to acquire mutations. The samples are to be subject to whole-genome sequencing. Ensuing sequence data will be subject to bioinformatic analyses to identify mutation signatures and their underlying causes.

本项目由威康信托基金（Wellcome Trust）通过战略奖立项资助，参与研究者包括史蒂夫·杰克逊（Steve Jackson）、迈克·斯特拉顿（Mike Stratton）、彼得·坎贝尔（Peter Campbell）、塞雷娜·尼克-扎伊纳尔（Serena Nik-Zainal）等。该拨款旨在资助相关研究工作，以识别由细胞DNA修复缺陷、其他细胞因子异常，或细胞暴露于各类DNA损伤剂所引发的「突变特征（mutational signatures）」。本次提供的样本源自酿酒酵母（Saccharomyces cerevisiae），涵盖野生型菌株，以及经过数月传代积累突变或未经过传代的各类基因敲除菌株。所有样本将接受全基因组测序（whole-genome sequencing），后续获得的测序数据将通过生物信息学分析，以鉴定突变特征及其潜在成因。