A proteomic view of artificially aged seeds of three rice lines of Kasalath with different anti-aging ability guided by miR164c

MicroRNAs (miRNAs) play important roles in various aspects of plant physiology and metabolism. The expression level of miR164c is negatively correlated with seed vigor in rice (Oryza sativa L.); however, the mechanism of seed vigor regulation by miR164c remains unknown. Here, we studied the proteomic difference between artificially aged seeds of the wild-type rice cultivar ‘Kasalath’ and its transgenic derivatives, miR164c-silenced line (MIM164c) and miR164c overexpression line (OE164c), with significant differences in anti-aging capacity. We hope that it can provide new insights into the mechanism of miRNA on regulating seed vigor.

微小RNA（miRNAs）在植物生理与代谢的诸多方面发挥着关键调控作用。在水稻（Oryza sativa L.）中，miR164c的表达水平与种子活力呈负相关，但miR164c调控种子活力的具体分子机制仍未明确。本研究以抗老化能力存在显著差异的水稻野生型品种‘Kasalath’及其转基因衍生株系——miR164c沉默株系（MIM164c）与miR164c过表达株系（OE164c）的人工老化种子为材料，开展蛋白质组学差异分析，旨在为解析miRNA调控种子活力的分子机制提供全新的研究视角。