Characterization of the NifA-RpoN Regulon in Rhizobium etli CFN42 in Free Life using whole genome transcript analysis

DNA microarray technology was used to survey changes in gene expression in R. etli CFN42 wild type compared with NifA CFNX247 mutant strain under microaerobic (free living) conditions. As expected, several genes associated with a NifA binding site were downregulated in the NifA mutant strain. Three independent biological materials with one dyeswap were performed.

本研究采用DNA微阵列（DNA microarray）技术，对微需氧（自由生活）条件下R. etli CFN42野生型菌株与NifA CFNX247突变菌株的基因表达（gene expression）变化进行了检测。正如预期，若干携带有NifA结合位点的基因在该NifA突变菌株中呈现表达下调。本次实验共设置3份独立生物学重复样本，并完成了1次染料互换（dyeswap）实验。