Sugarcane transcriptome - Drought response

Sugarcane one-eyed seed sets obtained from a variety sensitive to drought conditions (cv. SP90-1638, CTC, Brazil) were cultivated on moist sand for 15 days. The plants were transferred to pots containing moist sand and were irrigated with Hoagland’s solution (Hoagland and Arnon, 1950). Regular watering was maintained for 90 days and suppressed after this period for the experimental group. Leaves were collected 24 h, 72 h and 120 h after exposure to drought conditions for the control and experimental groups. Samples were collected and immediately frozen in liquid nitrogen. Leaves from six plantlets were used for each time point. Extraction of total RNA was performed separately on each sample pool. Keywords: time course of stress response RNA from experimental sample and from its respective control sample (untreated plants) was used for hybridization to dual channel arrays. Two biological replicates were used for each experimental point. The quantification of each hybridization was submitted in two files, one for each slide side (technical replicates).

本研究采用源自巴西CTC的干旱敏感型甘蔗品种SP90-1638所获得的单眼种茎，先将其置于湿润砂质基质中培养15天。随后将植株移栽至装有湿润砂质基质的盆钵内，以霍格兰德营养液（Hoagland's solution，Hoagland与Arnon，1950）进行浇灌。常规浇灌持续90天，此后实验组停止浇水以施加干旱胁迫。分别在对照组与实验组遭受干旱胁迫后的24 h、72 h及120 h时采集叶片样本，采集完成后立即将样本置于液氮中速冻保存。每个时间点的样本取自6株幼苗的叶片，随后分别对每个样本混合池开展总RNA提取。关键词：胁迫响应时序；实验样本及其对应对照样本（未处理植株）的RNA用于双通道基因芯片（dual channel arrays）杂交。每个实验点设置2次生物学重复，每次杂交的定量结果以两份文件提交，分别对应芯片玻片的两侧（技术重复）。