Transcriptome analysis of reticulated platelets reveals a prothrombotic profile [mRNA-Seq]

To compare immature and mature platelet transcriptome Methods: reticulated or immature platelets(RPs) were defined as the platelets with the highest thiazole orange staining intensity (15% highest, RNAhigh) according to previous experiences and mature platelets were defined as the platelet with the 30% lowest thiazole orange staining intensity (RNAlow) and sorted (supplemental Figure S1D). We detected more than 1700 differentially expressed gene, incluning activation receptors RPs have a prothrombotic transcriptomic profile Overall design: Platelets RNA was acquired frome healthy donors. And analyzed using using NextSeq-500为对比未成熟血小板与成熟血小板的转录组特征。方法：根据既往研究经验，将噻唑橙染色强度最高的15%血小板定义为网状血小板或未成熟血小板（reticulated or immature platelets, RPs），即RNA高表达组（RNAhigh）；将噻唑橙染色强度最低的30%血小板定义为成熟血小板（mature platelets），即RNA低表达组（RNAlow），并对两组血小板进行分选（补充图S1D）。本研究共检测到1700余个差异表达基因，其中包含活化受体；未成熟血小板具有促血栓形成的转录组特征。实验总体设计：从健康供体中获取血小板RNA，并使用NextSeq-500平台进行测序分析。