Complementies strategies encoded by DBD and IDRs direct TF binding

Profiling TF sequence variants to map their specificity determinants Overall design: 49 Transcription factors were tagged with MNAse, sequence variants constructed using CRIPSR and profiled using ChEC-Seq

对转录因子（Transcription Factor, TF）序列变异体开展谱学分析，以绘制其特异性决定位点。整体实验设计：为49个转录因子融合标记微球菌核酸酶（Micrococcal Nuclease, MNase），通过成簇规律间隔短回文重复序列（Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR）技术构建序列变异体，并采用染色质内源切割测序（Chromatin Endogenous Cleavage sequencing, ChEC-Seq）完成谱学分析。