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MinKNOW + Porechop nanopore reads

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DataCite Commons2022-02-02 更新2024-07-27 收录
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https://figshare.com/articles/dataset/MinKNOW_Porechop_nanopore_reads/6323099/1
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These files contain the nanopore long reads produced by R9.4 MinION, basecalled during sequencing by MinKNOW (v.Jun17) and demultiplexed by Porechop v0.2.1.<br>Half of each strain's sample underwent end repair, and half did not - these were sequenced under different barcodes. The samples here have had the reads from both barcodes pooled (see "Albacore + Porechop nanopore reads" for the individual readsets).<br><br>These reads were superseded by those from the more accurate basecaller, Albacore (see "Albacore only nanopore reads" for like-for-like equivalents).

本数据集文件包含由R9.4型MinION测序仪生成的纳米孔长读段(nanopore long reads),测序过程中通过MinKNOW软件(版本Jun17)完成碱基识别(basecalling),并由Porechop v0.2.1工具完成解多重索引。<br>每株菌株的样本中,一半进行了末端修复,另一半未进行末端修复;两组样本使用不同的测序标签(barcodes)进行了测序。本数据集的样本已将两种测序标签对应的读段进行合并(单独的读段集可参考"Albacore + Porechop纳米孔读段")。<br><br>该批读段已被更精准的碱基识别工具Albacore生成的读段所替代(一对一的等效数据集可参考"Albacore only纳米孔读段")。
提供机构:
figshare
创建时间:
2018-07-27
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