Expression data from Lbx1-EGFP+ flow sorted cells from Pitx2 WT, HT, Null forelimb tissue. Mus musculus

Determing the gene network regulated by Pitx2 in during forelimb muscle development of mouse embryos at E12.5. Lbx1 is coexpressed in Pitx2+ cells during forelimb development, thus Pitx2-LacZ and Lbx1-EGFP+ mice were cross bred to allow us to purify Lbx1-EGFP+|Pitx2 -wildtype, het, or null cells by flow sorting We used microarray analysis to determine the genes misregulated in Pitx2 null mice compared to Pitx2 wildtype and heterozyogtes. Overall design: Lbx1-EGFP+|Pitx2(+/+, LacZ/+, or LacZ/LacZ) cells were flow sorted, RNA extracted, labelled cDNA was hybridized to Affymetrix mouse genome 430 2.0 arrays

本数据集旨在探究小鼠胚胎E12.5时期前肢肌肉发育过程中受Pitx2调控的基因调控网络。在前肢发育过程中，Lbx1与Pitx2阳性细胞存在共表达特征，因此我们将Pitx2-LacZ品系小鼠与Lbx1-EGFP+品系小鼠进行杂交，从而可通过流式分选技术纯化得到同时表达Lbx1-EGFP，且Pitx2基因型为野生型、杂合型或纯合敲除型的细胞。我们采用基因芯片分析技术，对比Pitx2纯合敲除型小鼠与野生型、杂合型小鼠的基因表达谱，筛选出受Pitx2异常调控的基因。实验整体设计：对Lbx1-EGFP阳性且Pitx2基因型为(+/+、LacZ/+或LacZ/LacZ)的细胞进行流式分选，提取RNA并标记cDNA，随后将标记好的cDNA与Affymetrix小鼠全基因组430 2.0芯片进行杂交。