High maternal FA alters the expression of Slc17a7p and Vgll2p in the cerebral hemispheres of female newborn pups.

Total cell lysates from the cerebral hemispheres of female (F) newborn pups were prepared from both 0.4 mg/kg and 4 mg/kg FA diet groups and proteins were resolved on 10% Tris/HEPES/SDS-polyacrylamide gels. The blot was probed with Slc17a7p antibody (A, B) or with Vgll2p antibody (C, D). To confirm equal protein loading, the membranes were re-probed with GAPDH/β-tubulin antibodies. The left panel represents one representative blot and the right panel shows densitometric evaluation of two independent experiments. Densitometric evaluation of the bands were calculated by using the Image J software and normalized to the densities of GAPDH//β-tubulin. The values corresponding to 0.4 mg/kg FA diet were arbitrarily set to 100%, and the 4 mg/kg values are presented relative to this. The densitometric values represent the mean, and the error bar represents the inter-variability among independent experiments.

分别从0.4 mg/kg与4 mg/kg叶酸（FA）饮食组的雌性新生幼崽大脑半球中提取总细胞裂解液，随后将蛋白于10% Tris/HEPES/SDS-聚丙烯酰胺凝胶中进行电泳分离。本免疫印迹实验分别以Slc17a7p抗体（对应A、B组）与Vgll2p抗体（对应C、D组）进行杂交检测。为验证各组蛋白上样量一致，将转印膜重新以甘油醛-3-磷酸脱氢酶（GAPDH）/β-微管蛋白抗体进行杂交。左侧面板为单张代表性免疫印迹结果，右侧面板则展示两次独立实验的灰度定量分析结果。条带的灰度定量分析采用Image J软件完成，并以GAPDH/β-微管蛋白的灰度值为参照进行归一化处理。将0.4 mg/kg叶酸饮食组对应的灰度值人为设定为100%，4 mg/kg组的数值均以该基准进行相对呈现。所有灰度定量数值均以平均值表示，误差棒则代表各独立实验间的组间变异度。