Data_Sheet_2_Genome-Wide Identification of Sequence Variations and SSR Marker Development in the Munake Grape Cultivar.PDF

The Munake grape cultivar produces uniquely flavored and high-quality fruits. Despite the numerous beneficial agronomic traits of Munake, there are few genetic resources available for this cultivar. To address this knowledge gap, the entire genome was sequenced using whole-genome sequencing approaches and compared with a Vitis vinifera L. reference genome. This study describes more than 3 million single nucleotide polymorphism (SNP), 300,000 insertion and deletion (InDel), 14,000 structural variation (SV), and 80,000 simple sequence repeat (SSR) markers (one SSR per 4.23 kb), as well as their primers. Among the SSRs, 44 SSR primer pairs were randomly selected and validated by polymerase chain reaction (PCR), allowing discrimination between the different Munake cultivar genotypes. The genetic data provided allow a deeper understanding of Munake cultivar genomic sequence and contribute to better knowledge of the genetic basis behind its key agronomic traits.

穆纳克葡萄品种（Munake）可结出风味独特、品质优异的果实。尽管该品种具备诸多优良农艺性状，但目前针对其的可用遗传资源却极为稀缺。为填补这一研究空白，本研究采用全基因组测序方法对该品种的全基因组进行测序，并与欧洲葡萄（Vitis vinifera L.）参考基因组进行比对分析。本研究共鉴定得到超过300万个单核苷酸多态性（single nucleotide polymorphism, SNP）、30万个插入缺失（insertion and deletion, InDel）、1.4万个结构变异（structural variation, SV）以及8万个简单序列重复（simple sequence repeat, SSR）标记（平均每4.23 kb即存在1个SSR标记），并同步提供了对应的引物序列。其中随机选取44对SSR引物，通过聚合酶链式反应（polymerase chain reaction, PCR）进行验证，结果显示其可有效区分穆纳克葡萄品种的不同基因型。本研究提供的遗传数据有助于更深入地解析穆纳克葡萄品种的基因组序列，同时也为阐明其关键农艺性状背后的遗传基础提供了重要支撑。