UNESCO eDNA expedition in Brazilian Atlantic Islands: Fernando de Noronha and Atol das Rocas Reserves (Brazil): November 2022

This dataset contains the eDNA data collected as a part of the UNESCO eDNA expeditions in Marine World Heritage Sites project in 2022-2024. The project was coordinated in collaboration with the Ocean Biodiversity Information System (OBIS) of IOC-UNESCO and the marine programme of the UNESCO World Heritage Center, and funded by the government of Flanders (Kingdom of Belgium). The samples were collected with citizen science eDNA sampling kits, containing a syringe and a filter, and preserved with Longmire's buffer, before sequencing at a central laboratory. Five different biomarkers were analysed, including 12SMifish, Mimammal, Teleo, 16S-Vert and COI markers. The sequences were analysed with the PacMAN pipeline and quality controlled including a validation check by local scientists and site managers as well as compared to existing knowledge on species distributions in OBIS and GBIF. The full sampling protocols and materials are available on the project website (https://www.unesco.org/en/edna-training-materials?hub=66910), and the bioinformatics pipeline is on github (https://github.com/iobis/PacMAN-pipeline). The data can also be explored on the eDNA expeditions dashboard (https://dashboard.ednaexpeditions.org/).

该数据集包含2022-2024年联合国教科文组织（UNESCO）海洋世界遗产地环境DNA（eDNA）考察项目所采集的环境DNA数据。该项目由国际海洋学委员会-联合国教科文组织（IOC-UNESCO）下属的海洋生物多样性信息系统（OBIS）与联合国教科文组织世界遗产中心海洋项目协同合作开展，资金来源于比利时王国弗拉芒政府。样本通过公民科学环境DNA采样试剂盒采集，试剂盒包含注射器与滤器，样本经朗迈尔缓冲液（Longmire's buffer）保存后，送往中央实验室进行测序。研究分析了五种不同的生物标志物，包括12SMifish、Mimammal、Teleo、16S-Vert及COI标记物。序列通过PacMAN流程进行分析，并经过质量控制——包括由当地科学家与站点管理人员开展的验证检查，同时与OBIS和全球生物多样性信息机构（GBIF）中已有的物种分布知识进行对比。完整的采样方案与材料可在项目网站获取（https://www.unesco.org/en/edna-training-materials?hub=66910），生物信息学流程则发布于GitHub平台（https://github.com/iobis/PacMAN-pipeline）。该数据亦可通过环境DNA考察仪表板进行浏览（https://dashboard.ednaexpeditions.org/）。