Simulation of in vitro water deficit for selecting drought-tolerant banana genotypes

Abstract: The objective of this work was to determine a method to simulate water stress in vitro to select drought-tolerant banana (Musa sp.) genotypes. The BRS Tropical and Prata Anã cultivars were grown in vitro in an MS liquid medium containing sucrose and benzylaminopurine (BAP), supplemented with different concentrations of polyethylene glycol (PEG) or sorbitol. The yield stability index of dry matter was evaluated. Cultivation for 30 days in a medium containing 15 g L-1 PEG or 36.4 g L-1 sorbitol is appropriate to simulate water stress in vitro.

摘要：本研究旨在确立一种离体（in vitro）模拟水分胁迫的方法，以筛选耐旱香蕉（Musa sp.）基因型。选取BRS Tropical与Prata Anã两个香蕉品种，接种于含有蔗糖与苄氨基嘌呤（benzylaminopurine, BAP）的MS液体培养基中进行离体培养，并向培养基中添加不同浓度的聚乙二醇（polyethylene glycol, PEG）或山梨醇。随后对干物质产量稳定性指数进行了评估。结果表明，在添加15 g·L⁻¹ PEG或36.4 g·L⁻¹山梨醇的培养基中培养30天，适用于离体模拟香蕉的水分胁迫。