Human amnion epithelial cells: targeted protein profiling using Olink

These datasets contain data from quantification of proteins secreted by human amnion epithelial cells (AECs). Proteins were quantified using Olink's Target 96 Inflammation panel. Data are presented as normalized protein expression (NPX).Olink analysis 1: contains data from paired samples from 4 placenta donors on AEC-conditioned medium (AEC-CM) from 3 and 7 days of normoxic and hypoxic cultureOlink analysis 2: contains data on AEC-CM from 22 placenta donors, plus a medium control samples (cell culture medium not exposed to AECs).<br>For more info on analyzed samples, their analysis and our study setup, please see the publication below.<br>Zeijlon L, Budhwar S, Lindau R, Bencina S, Kaipe H, Jenmalm MC, Gramignoli R and Raffetseder J (2026). <b>Human amnion epithelial </b><b>cells induce M2 macrophage polarisation </b><b>partially via M-CSF secretion but </b><b>independently of extracellular vesicles </b><b><i>in vitro</i></b><b>.</b> Front. Immunol. 17:1723968. doi: 10.3389/fimmu.2026.1723968https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2026.1723968/<br><b>Abstract:</b>Pregnancy requires major immunomodulatory changes, both systemically and locally, as the maternal immune system needs to be modulated to tolerate the semi-allogeneic foetus. Decidual macrophages and stromal cells, but also foetal tissues are involved in this immune tolerance, for example by inducing M2 macrophages and regulatory T cells. However, it is so far unknown whether foetal membrane cells such as amnion epithelial cells (AECs) can influence human macrophage polarisation. In this study, a human in vitro macrophage assay was employed to demonstrate that conditioned medium (CM) from AECs derived from term placentas induces M2 macrophage polarisation, and to compare AEC culture conditions aiming for efficient M2 polarisation. Macrophage colony-stimulating factor (M-CSF), a well-known M2-inducing cytokine, was found to be secreted by AECs, and M-CSF was partly responsible for the observed M2-polarising effect of AECs. In addition, the M2-polarising effect remained after removal of extracellular vesicles (EVs) from AEC-CM, suggesting the involvement of soluble but not of EV-associated mediators. Taken together, this study shows that AECs may contribute to the induction of the vital immunotolerant environment at the foetal-maternal interface. Based on their immunomodulatory effects observed here and in in vivo studies, AECs could be harnessed as cytotherapeutics for inflammatory disorders.

本数据集包含人羊膜上皮细胞（human amnion epithelial cells, AECs）所分泌蛋白的定量检测数据。蛋白定量通过Olink Target 96炎症检测面板完成，数据以标准化蛋白表达量（normalized protein expression, NPX）形式呈现。
Olink分析1：包含4名胎盘供体的配对样本数据，对应常氧与低氧培养3天、7天的AEC条件培养基（AEC-conditioned medium, AEC-CM）。
Olink分析2：包含22名胎盘供体的AEC条件培养基数据，以及1份培养基对照样本（未接触AECs的细胞培养基）。
如需了解分析样本、检测流程及本研究设计的更多细节，请参阅下述文献：
Zeijlon L、Budhwar S、Lindau R、Bencina S、Kaipe H、Jenmalm MC、Gramignoli R及Raffetseder J（2026）。《人羊膜上皮细胞通过分泌巨噬细胞集落刺激因子部分诱导M2巨噬细胞极化，且在体外不依赖细胞外囊泡》。《免疫学前沿（Frontiers in Immunology）》，17卷：1723968。DOI: 10.3389/fimmu.2026.1723968，原文链接：https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2026.1723968/
摘要：
妊娠需要全身及局部的大规模免疫调节变化，因为母体免疫系统需被调控以耐受半同种异体胎儿。蜕膜巨噬细胞、基质细胞以及胎儿组织均参与该免疫耐受过程，例如通过诱导M2巨噬细胞与调节性T细胞实现。然而截至目前，尚不清楚羊膜上皮细胞（AECs）这类胎膜细胞是否可影响人巨噬细胞极化。本研究采用人体外巨噬细胞检测模型，证实足月胎盘来源AEC的条件培养基（conditioned medium, CM）可诱导M2巨噬细胞极化，并对比了可高效诱导M2极化的AEC培养条件。巨噬细胞集落刺激因子（macrophage colony-stimulating factor, M-CSF）作为经典的M2诱导型细胞因子，可由AECs分泌，且其部分介导了AECs所观察到的M2极化效应。此外，从AEC条件培养基中去除细胞外囊泡（extracellular vesicles, EVs）后，其M2极化效应仍可维持，提示该效应由可溶性介质而非囊泡相关介质所介导。综上，本研究表明AECs可参与构建胎儿-母体界面关键的免疫耐受微环境。基于本研究及体内研究中观察到的免疫调节效应，AECs可被开发为炎症性疾病的细胞治疗手段。