Rhodococcus erythropolis mono-culture in control groups

Raw data from three parallel transcriptomes of Rhodococcus erythropolis mono-culture in 0 mM Al3+.The quality of the extracted RNA was assessed using a Nanodrop 2000 and an Agilent 4200 Tape Station bioanalyzer. Only RNA samples with an RNA integrity value (RIN) ≥7 were selected for cDNA library construction. The cDNA library fragments were purified using the AMPure XP system to ensure a preferred length of 400-500 bp. The number of PCR cycles was adjusted to 15, and the final amplified library was quality checked using a Bioanalyzer 2100 system. Finally, an equimolar library was constructed using the Kapa-sybr FAST qPCR Kit Light Cycler 480 and a reference standard from Kapa Biosystems.
Each library was sequenced in paired-end mode using the TruSeq SBS kit v3-HS with a read length of 2 × 76 bp on the HiSeq2000 instrument (Illumina) according to the manufacturer's protocol for mRNA sequencing experiments. The R. erythropolis (GenBank assembly accession numbers: GCA_001715845.1) was used as the reference genome.

本数据集为0 mM铝离子浓度下，红球菌（Rhodococcus erythropolis）单培养样本的三份平行转录组原始数据。提取的总RNA质量通过Nanodrop 2000与安捷伦4200 Tape Station生物分析仪进行质控。仅选取RNA完整性值（RNA integrity value, RIN）≥7的RNA样本用于cDNA文库构建。使用AMPure XP系统对cDNA文库片段进行纯化，以确保目标片段长度为400~500 bp。将PCR循环数调整为15，随后通过Bioanalyzer 2100系统对最终扩增得到的文库进行质量验证。最后，使用Kapa-sybr FAST qPCR Kit、Light Cycler 480及Kapa Biosystems提供的参考标准品构建等摩尔浓度的文库。所有文库均按照Illumina官方的mRNA测序实验流程，采用TruSeq SBS kit v3-HS试剂盒，在HiSeq2000测序仪上以双端测序模式进行测序，读长为2×76 bp。本研究以红球菌（Rhodococcus erythropolis，GenBank组装登录号：GCA_001715845.1）作为参考基因组。