A Single Cell Transcriptome Atlas of The Domestic Pig. A Single Cell Transcriptome Atlas of The Domestic Pig

By leveraging single-cell/nuclei RNA sequencing, we generate the first single-cell transcriptome atlas of over 200,000 pig cells from 20 tissues/organs. Overall design: In this study, we report the generation of a single-cell transcriptome atlas of 222,526 cells across twenty tissues of the domestic pig by using scRNA-seq and single-nuclei RNA-sequencing (snRNAseq). Please note that the processed data files have been replaced and 3 samples (GSM6605687-GSM6605689) added on Sep 29, 2022. In the previous files, the mitochondria gene name in the gene matrix were with the "MT-" prefix. To be consistent with the pig Sus_scrofa11.1 genome assembly, the matrix has been updated with "MT-" prefix removed for the pig mitochondria genes.

本研究借助单细胞/细胞核RNA测序（single-cell/nuclei RNA sequencing）技术，构建了首个覆盖20种组织/器官、包含超20万个猪细胞的单细胞转录组图谱。
实验设计：本研究利用单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）与单细胞核RNA测序（single-nuclei RNA-sequencing, snRNAseq）技术，构建了覆盖家猪20种组织、包含222526个细胞的单细胞转录组图谱。请注意，本研究的处理后数据文件已完成更新替换，并于2022年9月29日新增3个样本（GSM6605687-GSM6605689）。在旧版数据文件中，基因矩阵内的线粒体基因名称带有"MT-"前缀。为与猪Sscrofa11.1基因组组装版本保持一致，本次更新已移除猪线粒体基因名称中的"MT-"前缀。