Genome-wide maps of ESR2 chromatin state in Mantle cell lymphoma cells

In order to elucidate chromatin binding regions of ESR2 in Mantle cell lymphoma, we used ESR2 antibodies, opti-mized chromatin-immunoprecipitation (ChIP), and performed ChIP-Seq of ESR2 over-expressed Granta-519 cells and Granta519-mock cells.We present for the first time, the cistrome of nuclear receptor ESR2 in Mantle cell lymphoma cell line. Examination of ESR2 chromatin binding in ESR2 over-expressed Granta-519 cells and Granta519-mock cells. Each ChIP was performed twice.

为阐明套细胞淋巴瘤（Mantle cell lymphoma）中雌激素受体2（Estrogen Receptor 2, ESR2）的染色质结合区域，我们采用ESR2抗体，优化了染色质免疫沉淀（chromatin-immunoprecipitation, ChIP）技术，并对过表达ESR2的Granta-519细胞与Granta519-mock细胞开展了ChIP测序（ChIP-Seq）。本研究首次报道了套细胞淋巴瘤细胞系中核受体ESR2的顺式调控组（cistrome）。我们对过表达ESR2的Granta-519细胞及Granta519-mock细胞中的ESR2染色质结合情况进行了检测。所有ChIP实验均重复两次。