The original gene expression data including four tissue from The role of three heat shock protein genes in the immune response to Aeromonas hydrophila challenge in marbled eel, Anguilla marmorata

Heat shock proteins (HSPs) are highly conserved molecular chaperones that play critical roles in both innate and adaptive immune. However, little information about hsps from marbled eel, Anguilla marmorata is known. In this study, the full-length Amhsp90 (2527 bp), Amhsp70 (2443 bp) and Amhsc70 (2247 bp) were first cloned from A. marmorata, using rapid amplification of cDNA ends, containing open reading frames with 2181, 1932 and 1950 bp in length, and encoding proteins with 726, 643 and 649 amino acids, respectively. The deduced amino acid sequences of three Amhsps shared a high homology similarity with other migratory fish. Real-time fluorescent quantitative-PCR was used to evaluate tissue-specific distribution and mRNA expression levels of three Amhsps subjected to the infection with Aeromonas hydrophila. The mRNA expression of three Amhsps in eight tested tissues, including liver, heart, muscle, gill, spleen, kidney, brain and intestine tissues of juvenile A. marmorata was evaluated to reveal the major expression distribution in liver, intestine, muscle and heart. After pathogen challenge treatments, mRNA transcriptions of three Amhsps revealed a significant regulation at various time points in the same tissue. All these findings suggest that Amhsps may be involved in the immune response in A. marmorata.

热休克蛋白（Heat shock proteins, HSPs）是一类高度保守的分子伴侣，在先天免疫与适应性免疫中发挥关键作用。然而目前关于花鳗鲡（Anguilla marmorata）的热休克蛋白的相关研究信息尚且匮乏。本研究采用cDNA末端快速扩增技术（rapid amplification of cDNA ends），首次从花鳗鲡中克隆得到全长为2527 bp的Amhsp90、2443 bp的Amhsp70以及2247 bp的Amhsc70；三者分别包含长度为2181 bp、1932 bp与1950 bp的开放阅读框，编码的蛋白质分别含有726、643和649个氨基酸残基。经推导得到的三种花鳗鲡热休克蛋白的氨基酸序列与其他洄游性鱼类具有较高的同源性。本研究采用实时荧光定量PCR技术，评估了嗜水气单胞菌（Aeromonas hydrophila）感染条件下三种Amhsps的组织特异性分布特征及mRNA表达水平：通过检测幼龄花鳗鲡的8种受试组织（肝脏、心脏、肌肉、鳃、脾脏、肾脏、脑组织与肠道）中的三种Amhsps的mRNA表达情况，发现其主要表达于肝脏、肠道、肌肉及心脏组织；经病原菌攻毒处理后，三种Amhsps的mRNA转录水平在同一组织的不同时间点均呈现显著调控变化。上述研究结果表明，Amhsps可能参与花鳗鲡的免疫应答过程。