Arginine-mediated dissociation of single cells and cell sheets from a polystyrene culture dish

Here, we report a novel non-enzymatic cell dissociation method, based on our finding that adherent cells dissociate rapidly from the polystyrene culture dish when incubated in an l- or d-arginine-containing solution. We also demonstrate the successful detachment of confluent NIH/3T3 cell monolayers from the culture dish as a cell sheet by the addition of an arginine solution.

本研究报道了一种新型非酶解细胞解离方法，其研发基于本团队的发现：当贴壁细胞在含L-精氨酸（L-arginine）或D-精氨酸（D-arginine）的溶液中孵育时，可快速从聚苯乙烯培养皿表面脱离。本研究同时证实，通过添加精氨酸溶液，可成功将处于汇合状态的NIH/3T3细胞单层以细胞片层的形式从培养皿上完整脱离。