Genome-wide polysome analysis in poly(I:C)-treated WT and Oasl1-/- BMDM.

OASL1 is a novel translation inhibitor for the type I IFN master transcription factor IRF7. To examine whether OASL1 specifically inhibit IRF7 translation, we used a genome-wide gene expression microarray to screen for the polysome-associated mRNAs more enriched in the OASL1 KO BMDMs. The microarray analysis on the polysomal fractions identified 145 candidate transcripts, including IRF7, that showed a signal greater than two-fold higher in poly(I:C)-treated Oasl1-/- BMDM. We analyzed polysomal fractions from poly(I:C)-treated WT and Oasl1-/- BMDM using the Affymetrix Mouse Gene 1.0 ST array. Array data were processed by Affymetrix GCOS software. We compared these using Affymetrix Expression console1.1, R affy-package(2.9.2), DAVID.

OASL1是一种靶向I型干扰素（type I IFN）核心转录因子干扰素调节因子7（IRF7）的新型翻译抑制剂。为验证OASL1是否可特异性抑制IRF7的翻译过程，我们采用全基因组基因表达芯片，筛选在OASL1敲除（KO）骨髓源性巨噬细胞（BMDMs）中富集程度更高的多聚核糖体结合mRNA。对多聚核糖体组分的芯片分析共鉴定出145个候选转录本，其中包括IRF7，这些转录本在经poly(I:C)处理的Oasl1纯合敲除（Oasl1-/-）骨髓源性巨噬细胞中的信号强度较对照组高出2倍以上。我们使用Affymetrix Mouse Gene 1.0 ST芯片，对经poly(I:C)处理的野生型（WT）与Oasl1-/-骨髓源性巨噬细胞的多聚核糖体组分进行了分析。芯片数据经Affymetrix GCOS软件处理后，我们使用Affymetrix Expression Console 1.1、R语言affy包（2.9.2）以及DAVID工具对其进行了比对分析。