Comparison of neural stem cell lines overexpressing Nurr1 to clonal controls

The orphan nuclear receptor Nurr1 has been shown to be critical for the development of ventral midbrain dopaminergic neurons. Consequently, the development of ES cells overexpressing Nurr1 has raised hope for the development of cell replacement therapies for Parkinson's Disease to replace degenerated dopaminergic neurons. However, the molecular consequences of Nurr1 on gene expression in these cells remain unknown. To address this, stable, clonal, c17.2 neural stem cell lines were established that overexpressed the orphan nuclear receptor Nurr1 (clone 42 & clone 48) or parental control cell line (puroB & puroD, respectively). Keywords: genetic modification Stable neural stem cell lines were grown in proliferating conditions and matched for further microarray analysis based on their similar proliferation rates: clone 42(c42) vs. puroB(pB) clone 42(c48) vs. puroD(pD)

已有研究证实，孤儿核受体（orphan nuclear receptor）Nurr1对腹侧中脑多巴胺能神经元的发育至关重要。因此，过表达Nurr1的胚胎干细胞（embryonic stem cells, ES细胞）的构建，为帕金森病（Parkinson's Disease）的细胞替代疗法带来了希望——该疗法旨在替换变性丢失的多巴胺能神经元。然而，目前尚不清楚Nurr1对这些细胞内基因表达的分子调控效应。为解决这一科学问题，本研究构建了稳定的克隆c17.2神经干细胞系：分别为过表达孤儿核受体Nurr1的克隆42（clone 42）与克隆48（clone 48），以及各自对应的亲本对照细胞系puroB与puroD。关键词：基因修饰（genetic modification）。将稳定神经干细胞系置于增殖条件下培养，并根据其相似的增殖速率进行配对，以用于后续的微阵列（microarray）分析：克隆42（c42）与puroB（pB）配对，克隆48（c48）与puroD（pD）配对。