Coordinated regulation of polycomb group complexes through microRNAs in cancer

To determine the altered mRNA expression signatures upon overexpressing EZH2 regulated microRNAs in cancer. To identify EZH2 regulated microRNAs in cancer. Two-condition experiment: Each EZH2 microRNA was overexpressed and compared to a control RNA in BT549 breast cancer cells. In DU145 prostate cancer cell EZH2 was knocked down and comapred to EZH2 scrambled siRNA. Other cell lines were monitored for miRNA expression as they are known to have high endogenous levels of EZH2.

本研究旨在明确癌症中受EZH2调控的微小RNA（microRNA）过表达后mRNA表达特征的变化，并鉴定癌症中受EZH2调控的微小RNA。本实验采用双条件实验设计：在BT549乳腺癌细胞中，对每一种受EZH2调控的微小RNA进行过表达处理，并以对照RNA作为对照进行比对；在DU145前列腺癌细胞中，敲低EZH2的表达，并以转染EZH2阴性对照小干扰RNA（scrambled siRNA）的细胞作为对照进行比对。此外，由于已知其他细胞系的内源性EZH2表达水平较高，因此对这些细胞系的微小RNA表达情况进行了检测。