Discovering new pneumococcal diagnostic tools protein-based

Thanks to surface digestion protocol above pneumococcal pediatric strains and in silico selection, we selected 94 pneumococcal proteins according to their surface-exposed domains and/or interactions with surface environment. Then, that proteins were cloned as recombinant form in Escherichia coli and face off to pediatric sera from control and pneumococcal patients.

借助针对儿童肺炎链球菌菌株的表面消化方案以及计算机虚拟筛选（in silico selection），我们依据蛋白的表面暴露结构域以及与表面环境的相互作用，筛选得到94种肺炎链球菌蛋白。随后，我们将这些蛋白以重组形式在大肠埃希菌（Escherichia coli）中克隆，并与对照组及肺炎患者的儿童血清进行了血清学反应实验。