ATAC-seq chromatin accessibility profiling in pancreatic islets isolated from Arid1aFl/Fl ; MIP-rtTA control and Arid1aFl/Fl; MIP-rtTA; TRE-Cre mice (Arid1a βKO) with/without pancreatectomy. ATAC-seq chromatin accessibility profiling in pancreatic islets isolated from Arid1aFl/Fl ; MIP-rtTA control and Arid1aFl/Fl; MIP-rtTA; TRE-Cre mice (Arid1a βKO) with/without pancreatectomy

To determine how ARID1A loss might regulate genes that were preferentially regulated on the gene expression level, we performed an assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) on islets isolated from control and Arid1a β-cell knockout (βKO) mice at baseline and 6 days after 50% pancreatectomy (PPx). Overall design: In vivo ATAC-Seq on islets isolated from 3-month-old male control and Arid1a βKO mice at baseline and 6 days after PPx (Pre-PPx: 4 Arid1aFl/Fl; MIP-rtTA control and 3 Arid1aFl/Fl; MIP-rtTA; TRE-Cre mice, Post-PPx: 4 Arid1aFl/Fl; MIP-rtTA control and 4 Arid1aFl/Fl; MIP-rtTA; TRE-Cre mice, all males).

为明确ARID1A缺失对基因表达层面优先调控基因的调控机制，我们针对基线状态及50%胰腺切除术（PPx）后6天的对照组与Arid1a β细胞敲除（βKO）小鼠分离的胰岛，开展了转座酶可及性染色质高通量测序（ATAC-seq）实验。实验整体设计：于3月龄雄性对照组与Arid1a βKO小鼠的分离胰岛中进行体内ATAC-seq检测，采样时点分为基线状态与PPx后6天；其中PPx前样本含4只Arid1aFl/Fl; MIP-rtTA对照小鼠与3只Arid1aFl/Fl; MIP-rtTA; TRE-Cre小鼠，PPx后样本含4只Arid1aFl/Fl; MIP-rtTA对照小鼠与4只Arid1aFl/Fl; MIP-rtTA; TRE-Cre小鼠，所有受试个体均为雄性。