Replication Data for Changes of RAS pathway phosphorylation in lymphoblastoid cell lines from Noonan syndrome patients carrying hypomorphic variants in two NS genes

This dataset include the LC-MS/MS proteomics and phosphoproteomics datasets of changed proteins generated in the study: "Changes of RAS pathway phosphorylation in lymphoblastoid cell lines from Noonan syndrome patients carrying hypomorphic variants in two NS genes" by Viviana Tritto, Daniele Capitanio, Cecilia Gelfi and Paola Riva. Data were generated by LC-ESI-MS/MS with label-free quantitation. Mass spectra were analyzed using MaxQuant software (Max-Planck-Institute of Biochemistry, Munich, Germany, version 1.6.3.3). Statistical analyses were performed using the Perseus software (Max Planck Institute of Biochemistry, Munich, Germany, version 1.4.0.6). For statistical analysis, ANOVA and Tukey post-hoc test with a p-value threshold of 0.05 was applied. To exclude the presence of false positives from the analysis, Benjamini–Hochberg false discovery rate test was applied.

本数据集包含Viviana Tritto、Daniele Capitanio、Cecilia Gelfi及Paola Riva开展的研究“携带两个NS基因亚效变异的Noonan综合征患者淋巴母细胞系中RAS通路磷酸化变化”中产生的差异蛋白质的LC-MS/MS蛋白质组学与磷酸化蛋白质组学数据。数据通过LC-ESI-MS/MS技术结合无标记定量法生成，质谱数据采用MaxQuant软件（德国慕尼黑马克斯·普朗克生物化学研究所，版本1.6.3.3）分析，统计分析使用Perseus软件（德国慕尼黑马克斯·普朗克生物化学研究所，版本1.4.0.6）完成。统计分析中应用ANOVA检验及Tukey事后检验，p值阈值设定为0.05；为排除假阳性结果，进一步采用Benjamini–Hochberg假发现率检验。