Effect of twenty-one different nitrogen sources on global gene expression in the yeast Saccharomyces cerevisiae. Saccharomyces cerevisiae

We have applied whole-genome microarray hybridization to compare the transcriptome of wild-type yeast strain S1278b during growth on a minimal medium containing 21 different single nitrogen sources including urea used as a reference condition. Keywords: growth conditions comparison Overall design: The 21 compounds were selected from a list of 27 nitrogen sources for their ability to support reproducible growth with a generation time (g) of less than 5 hours. The reference nitrogen source to which the twenty other media were compared in terms of genome expression was urea, chosen because urea catabolism and its regulation are well known, because the generation time of strain S1278b on urea is near the middle of the studied g range, and because the major regulations like nitrogen catabolite repression (NCR) and SPS-mediated control are not operational on this medium.

本研究采用全基因组微阵列杂交（whole-genome microarray hybridization）技术，对野生型酵母菌株S1278b在含有21种不同单一氮源的基础培养基中生长时的转录组（transcriptome）进行比较分析，其中以尿素作为参照条件。
关键词：生长条件比较
整体设计：本研究从27种氮源中筛选出21种，这些氮源可支持菌株稳定且可重复的生长，且代时（generation time，g）小于5小时。本研究以尿素作为其余20种培养基在全基因组表达层面的参照氮源，选取尿素作为参照的原因如下：其一，尿素的分解代谢及其调控机制已被充分阐明；其二，酵母菌株S1278b在尿素培养基中的代时处于本次研究所覆盖代时范围的中间位置；其三，氮分解代谢阻遏（nitrogen catabolite repression，NCR）与SPS介导的调控（SPS-mediated control）等主要调控通路在尿素培养基中均不发挥作用。