Genome sequencing of two strains of Bifidobacterium longum subspecies infantis causing bacteremia in neonates and six control strains. Bifidobacterium longum subsp. infantis

Administration of probiotics to premature newborns prevents necrotizing enterocolitis and reduces all cause mortality. Two cases of Bifidobacterium infantis bacteremia were diagnosed in newborns receiving probiotics. This study describes the sequence of the two strains causing bacteremia retrieved by blood culture and six other strains isolated directly from probiotics capsules. DNA was sequenced on an Illumina MiSeq system with a 2x150 bp run. SNP analysis was performed to confirm the probiotic origin of the B. infantis strain recovered from neonates with bacteremia.

向早产儿施用益生菌可预防坏死性小肠结肠炎，并降低全因死亡率。在接受益生菌治疗的新生儿中，曾确诊2例婴儿双歧杆菌（Bifidobacterium infantis）菌血症病例。本研究对经血培养分离得到的两株致菌血症菌株，以及直接从益生菌胶囊中分离获得的另外6株菌株的序列进行了描述。采用Illumina MiSeq测序系统，以2×150 bp双端测序模式完成DNA测序。通过单核苷酸多态性（Single Nucleotide Polymorphism, SNP）分析，确认了从菌血症新生儿体内分离得到的婴儿双歧杆菌菌株的益生菌来源。