Characterization of Canine CD34+/CD45 diminished Cells by Colony-forming Unit Assay and Transcriptome Analysis

In veterinary medicine, peripheral blood stem cells have been successfully used for haematopoietic stem cell transplantation, especially in cases of canine B-cell and T-cell lymphoma. However, the cell surface antigens and gene expression profiles of canine Haematopoietic stem and progenitor cells (HSPCs) are yet to be elucidated. The goals of this project are attempted to characterize the canine HSPCs using colony-forming unit assay and RNA sequencing analysis. We compared the haematopoietic colony forming abilities and HSPC-specific cell surface antigen gene expressions among whole viable cells (control), CD34+ cells, and CD34+/CD45dim cells. We believe that our study makes a significant contribution to the literature because our observations highlight the potential of the CD34+/CD45dim cells to be used as markers for studying canine haematopoietic stem cells. Further, we believe that this paper will help to exclusively identify the canine HSPCs, which, in turn, will help to develop treatment options for canine patients suffering from haematopoietic tumour diseases.

在兽医学领域，外周血干细胞已成功应用于造血干细胞移植，尤其在犬B细胞淋巴瘤与T细胞淋巴瘤的诊疗中。然而，犬造血干祖细胞（Haematopoietic Stem and Progenitor Cells, HSPCs）的细胞表面抗原特征与基因表达谱仍有待阐明。本研究旨在通过集落形成单位试验（Colony-Forming Unit Assay）与RNA测序分析，对犬HSPCs进行系统特征鉴定。我们比较了全活细胞（对照组）、CD34阳性细胞以及CD34+/CD45dim细胞的造血集落形成能力与HSPC特异性细胞表面抗原基因表达水平。我们认为本研究对相关学术文献具有重要贡献，因为本研究结果凸显了CD34+/CD45dim细胞作为犬造血干细胞研究标志物的应用潜力。此外，我们相信本研究将助力特异性鉴定犬HSPCs，进而为罹患造血系统肿瘤疾病的犬类患者开发治疗方案提供依据。