NiPPeR Study - pilot microbiome investigation

The NiPPeR double-blinded randomized controlled trial recruited 1,729 women prior to conception and followed 585 mother-infant dyads from delivery to examine the impact of a nutritional supplement [containing additional micronutrients, myo-inositol and probiotics - Lactobacillus rhamnosus NCC 4007 (CGMCC 1.3724; LPR) and Bifidobacterium animalis sp. lactis NCC 2818 (CNCM I-3446; Bl818)] taken before and during pregnancy on the health of mothers and their babies. In this pilot study, we analyzed 20 stool samples, collected six months after birth, from 10 mother-infant dyads within the NiPPeR cohort (five from Auckland, New Zealand; five from Southampton, United Kingdom) to characterize vertical mother-to-infant microbial transmission and subsequent adaptation in bacterial transcription.

NiPPeR双盲随机对照试验（double-blinded randomized controlled trial）共招募1729名孕前女性，对其中585个母婴二人组（mother-infant dyads）自分娩起开展随访，以探究孕前及孕期服用的营养补充剂对母婴健康的影响。该营养补充剂含有额外微量营养素、肌醇（myo-inositol）以及两种益生菌——鼠李糖乳杆菌（Lactobacillus rhamnosus）NCC 4007（CGMCC 1.3724，简称LPR）和动物双歧杆菌乳亚种（Bifidobacterium animalis subsp. lactis）NCC 2818（CNCM I-3446，简称Bl818）。本项先导性研究（pilot study）对NiPPeR队列（cohort）中的10个母婴二人组的20份于婴儿出生6个月后采集的粪便样本展开分析，其中5份样本采集自新西兰奥克兰，另5份采集自英国南安普顿，旨在表征母婴垂直微生物传播过程及细菌转录层面的后续适应性特征。