A class of secreted mammalian peptides with potential to expand cell-cell communication - Capped peptide detection

Using a hybrid computational-biochemical approach, we have demonstrated the endogenous presence of a large and sequence diverse class of orphan, blood-borne peptides that we call “capped peptides.” Capped peptides are post-translationally modified fragments of classically secreted proteins. They exhibit structural and regulatory characteristics similar to other signaling peptides, including N-terminal pyroglutamylation, C-terminal amidation, and cell type-specific expression. Circulating levels of capped peptides are dynamically regulated by internal physiologic states and external environmental stimuli.

The data deposited here are the raw .d files from the initial detection of the capped peptides in mouse and human plasma, along with synthetic standards. We are also including the files from evaluating the peptides level under different physiologic conditions: fasting, high fat diet, LPS injection, aging, and exercise.

本研究采用计算-生化混合研究方法，证实了一类内源性存在、序列多样性丰富的孤儿型血液肽类的存在，我们将其命名为“封端肽（capped peptides）”。封端肽为经典分泌蛋白经翻译后修饰产生的片段，其结构与调控特性与其他信号肽相似，具备N端焦谷氨酰化、C端酰胺化修饰以及细胞类型特异性表达等特征。封端肽的循环水平可随机体内部生理状态与外部环境刺激发生动态变化。

本数据集上传的原始数据包括小鼠与人体血浆中首次检测封端肽时得到的原始.d格式文件，以及合成肽标准品相关数据。此外还包含在不同生理条件下评估该肽类水平的相关文件，涉及禁食、高脂饮食、脂多糖（LPS）注射、衰老与运动干预等多种场景。