Genome-wide localization profile (ChIP-seq) and differential expression profile upon RNAi treatment (RNA-seq) of L(3)mbt and its interactors in ovarian somatic cells[OSCs] in Drosophila

L(3)mbt regulate the transcription of germ-specific genes in somatic cells but the detailed mechanism remains unclear. We performed ChIP-seq of L(3)mbt and a newly identified interactor in OSCs to elucidate the genome-wide localization. Then we performed RNA-seq of OSCs before and after RNAi treatment of L(3)mbt and its interactors and performed differential expression analysis. Overall design: ChIP-seq of OSCs and RNA-seq of OSCs before and after RNAi treatment of L(3)mbt and its interactors were performed in duplicate.

L(3)mbt 可调控体细胞内生殖特异性基因的转录，但其详细作用机制仍未阐明。我们针对卵巢体细胞干细胞（Ovary Somatic Cells, OSCs）中的L(3)mbt及其新鉴定的互作蛋白，开展了染色质免疫共沉淀测序（Chromatin Immunoprecipitation sequencing, ChIP-seq），以解析其全基因组定位特征。随后，我们对L(3)mbt及其互作蛋白实施RNA干扰（RNA interference, RNAi）处理，分别采集处理前后的OSCs进行转录组测序（RNA Sequencing, RNA-seq），并完成差异表达分析。整体实验设计：本研究针对OSCs开展了ChIP-seq实验，并对经L(3)mbt及其互作蛋白RNAi处理前后的OSCs进行了RNA-seq实验，所有实验均设置两次生物学重复。