DataSheet_14_Exploratory metabolomic analysis for characterizing the metabolic profile of the urinary bladder under estrogen deprivation.docx

BackgroundEstrogen homeostasis is crucial for bladder function, and estrogen deprivation resulting from menopause, ovariectomy or ovarian dysfunction may lead to various bladder dysfunctions. However, the specific mechanisms are not fully understood.
MethodsWe simulated estrogen deprivation using a rat ovariectomy model and supplemented estrogen through subcutaneous injections. The metabolic characteristics of bladder tissue were analyzed using non-targeted metabolomics, followed by bioinformatics analysis to preliminarily reveal the association between estrogen deprivation and bladder function.
ResultsWe successfully established a rat model with estrogen deprivation and, through multivariate analysis and validation, identified several promising biomarkers represented by 3, 5-tetradecadiencarnitine, lysoPC (15:0), and cortisol. Furthermore, we explored estrogen deprivation-related metabolic changes in the bladder primarily characterized by amino acid metabolism imbalance.
ConclusionThis study, for the first time, depicts the metabolic landscape of bladder resulting from estrogen deprivation, providing an important experimental basis for future research on bladder dysfunctions caused by menopause.

背景：雌激素稳态对于膀胱功能至关重要，而绝经、卵巢切除或卵巢功能障碍引发的雌激素剥夺，可能导致多种膀胱功能异常。然而，其具体作用机制尚未完全阐明。
方法：本研究通过大鼠卵巢切除模型模拟雌激素剥夺状态，并通过皮下注射方式补充雌激素。采用非靶向代谢组学分析膀胱组织的代谢特征，随后辅以生物信息学分析，初步揭示雌激素剥夺与膀胱功能之间的关联。
结果：本研究成功构建了雌激素剥夺大鼠模型，经多变量分析与验证，鉴定出以3,5-十四碳二烯肉碱（3,5-tetradecadiencarnitine）、溶血磷脂酰胆碱(15:0)（lysoPC (15:0)）及皮质醇（cortisol）为代表的多个潜在生物标志物。此外，本研究探明雌激素剥夺引发的膀胱代谢变化主要以氨基酸代谢失衡为特征。
结论：本研究首次描绘了雌激素剥夺所致膀胱组织的代谢全景，为后续绝经相关膀胱功能异常的研究提供了重要实验依据。