Histone modification (H3, H3K9ac, H3K9me2) ChIP-seq in WT and cbf11KO strains of Schizosaccharomyces pombe

Acetylation and dimethylation of lysine 9 on histone H3 are prominent marks of euchromatin and heterochromatin, respectively. Moreover, histone acetylation has been linked to lipid metabolism. We have previously identified the transcription factor Cbf11 as a regulator of lipid metabolism and genome integrity in the fission yeast. Cut6, the acetyl-CoA carboxylase, is one of direct targets of Cbf11. To link the role of Cbf11 in lipid metabolism and chromatin regulation we have performed ChIP-seq of H3K9ac, H3K9me2 and H3 in WT and cbf11KO strains of Schizosaccharomyces pombe in three biological replicates. Strain genotypes: wild type JB32 (h+s); cbf11 knock-out MP44 (h+ cbf11::natR).

组蛋白H3第9位赖氨酸的乙酰化与二甲基化，分别为常染色质与异染色质的典型表观遗传标记。此外，组蛋白乙酰化已被证实与脂质代谢存在关联。我们此前已在裂殖酵母中鉴定出转录因子Cbf11，其可作为脂质代谢与基因组完整性的调控因子。乙酰辅酶A羧化酶Cut6是Cbf11的直接靶标之一。为了关联Cbf11在脂质代谢与染色质调控中的作用，我们针对粟酒裂殖酵母（Schizosaccharomyces pombe）的野生型（WT）与cbf11敲除菌株，开展了针对H3K9ac、H3K9me2及H3的染色质免疫共沉淀测序（ChIP-seq）实验，共设置三次生物学重复。菌株基因型信息如下：野生型JB32（基因型为h+s）；cbf11敲除菌株MP44（基因型为h+ cbf11::natR）。