The effect of beta-glucan - induced trained immunity on the transcriptomic profile of tumor-associated neutrophils (TAN)

Induction of trained immunity by beta-glucan has been shown to promote transcriptomic alterations in myeloid cells. Neutrophils are involved in the immune response against tumors and can promote or inhibit tumor growth. In this study, we performed RNA sequencing in TAN from mice in order to investigate the impact of trained immunity on the transcriptomic profile of neutrophils in the tumor setting. Overall design: WT mice were pretreated with a single dose of 1 mg of ß-glucan from Trametes versicolor (Invivogen) in PBS or with PBS vehicle alone (control). After 7 days, mice were injected subcutaneously with 3 x 105 B16-F10 melanoma cells into the right flank. Neutrophils (CD45+CD11c-CD11b+Ly6c-Ly6g+) that were infiltrated in the tumor were sorted 14 days after tumor cell injection and RNA sequencing analysis was performed.

β-葡聚糖（beta-glucan）诱导的训练免疫（trained immunity）已被证实可促进髓系细胞的转录组改变。中性粒细胞参与抗肿瘤免疫应答，既可促进也可抑制肿瘤生长。本研究针对小鼠肿瘤相关中性粒细胞（tumor-associated neutrophils, TAN）开展RNA测序（RNA sequencing），以探究训练免疫对肿瘤微环境中中性粒细胞转录组特征的影响。实验设计概述：野生型（wild type, WT）小鼠经单次预处理，分别给予1 mg云芝（Trametes versicolor）来源的β-葡聚糖（由Invivogen公司提供）的PBS混悬液，或仅注射PBS溶剂作为对照组。7天后，于小鼠右侧腹侧皮下接种3×10^5个B16-F10黑色素瘤细胞。肿瘤细胞接种14天后，分选肿瘤浸润性中性粒细胞（CD45+CD11c-CD11b+Ly6c-Ly6g+），并进行RNA测序分析。