RNA-sequencing of WT and P2Y4 KO pericardial adipose tissue (PAT) 24 hours after left anterior descending artery (LAD) ligation

We identified differentially regulated genes in the pericardial adipose tissue of wild type (WT) and P2Y4 KO mice, 24 hours after left anterior descending artery (LAD) ligation as a model of myocardial infarction (MI). Differentially regulated genes were involved in metabolism, brown fat cell differentiation, immune response and inflammation. P2Y4 KO mice displayed adipocyte beiging with increased PD-L1 expression and a higher number of regulatory leukocytes in their PAT 24 hours after LAD ligation. Up-regulated genes linked to adipocyte beiging included Ucp1, Cited1 and Prdm16. Overall design: 2 samples were analyzed, a pool of the RNA of 3 WT PAT and a pool of the RNA of 3 P2Y4 KO PAT. PAT was freshly harvested 24 hours after LAD ligation.

本研究以左前降支（left anterior descending artery, LAD）结扎构建心肌梗死（myocardial infarction, MI）模型，于术后24小时，对野生型（wild type, WT）与P2Y4敲除（P2Y4 KO）小鼠的心包脂肪组织（pericardial adipose tissue, PAT）内的差异表达基因进行了鉴定。所鉴定出的差异表达基因参与代谢、棕色脂肪细胞分化、免疫应答及炎症反应等生物学过程。P2Y4敲除小鼠在左前降支结扎术后24小时，其心包脂肪组织中出现脂肪细胞米色化现象，PD-L1表达水平升高，调节性白细胞数量显著增多。与脂肪细胞米色化相关的上调基因包括Ucp1、Cited1及Prdm16。实验设计：本研究共分析2组样本，分别为3只野生型小鼠心包脂肪组织RNA的混合样本，以及3只P2Y4敲除小鼠心包脂肪组织RNA的混合样本；所有心包脂肪组织均于左前降支结扎术后24小时新鲜采集。