16S rRNA gene amplicon-based gut microbiome signature in cerebral vasospasm and delayed cerebral ischemia after aneurysmal subarachnoid hemorrhage: A prospective nested case-control pilot study

A prospective nested case-control study with 1:1 matching was conducted recruiting subjects with aSAH: cases with CVS; and controls without CVS based on the clinical picture and structured bedside transcranial Doppler (TCD). Fecal samples for microbiota analyses by means of 16S rRNA gene amplicon sequencing were collected within the first 96 hours after ictus.

本研究为一项采用1:1匹配设计的前瞻性巢式病例对照研究，纳入动脉瘤性蛛网膜下腔出血（aneurysmal subarachnoid hemorrhage, aSAH）受试者，依据临床特征及结构化床旁经颅多普勒（transcranial Doppler, TCD）检查结果划分病例组（合并脑血管痉挛（cerebral vasospasm, CVS）者）与对照组（无脑血管痉挛者）。本研究于受试者发病后96小时内采集粪便样本，通过16S rRNA基因扩增子测序技术开展微生物组分析。