Cortical transcriptomic changes in the newborn rat brain after exposure to surgery and anesthesia in the third trimester. Cortical transcriptomic changes in the newborn rat brain after exposure to surgery and anesthesia in the third trimester

We report changes in the cortical transcriptome of newborn rat pups after exposure to anesthesia and surgery in the third trimester using unbiased RNA-seq analysis. Overall design: We performed RNA-seq analysis to examine differential gene expression in the cortex of newborn pups (day 0) after in utero exposure to anesthesia and subcutaneous pump implantation surgery on embryonic day 18 (n = 2 pups each of either sex from 3 independent dams) or no treatment (2 pups each of either sex from 3 independent dams). With an FDR adjusted p < 0.05, none of the genes were determined to be significantly differentially expressed in the newborn brain after exposure to anesthesia and surgery in the third trimester.

本研究采用无偏倚RNA测序（RNA-seq）分析，报道了妊娠晚期暴露于麻醉与手术环境下的新生大鼠幼崽大脑皮层转录组的变化。
整体实验设计：我们对妊娠第18天接受宫内麻醉及皮下泵植入手术的新生幼崽（出生后第0天，雌雄各2只，来自3只独立孕鼠），以及未接受任何处理的对照组新生幼崽（雌雄各2只，来自3只独立孕鼠）的大脑皮层开展RNA-seq分析，以检测差异基因的表达情况。
经错误发现率（False Discovery Rate，FDR）校正后的P值<0.05的筛选标准判定，妊娠晚期暴露于麻醉与手术的新生大鼠大脑皮层中未发现显著差异表达的基因。