A.thaliana seedling expression data from ASP treatment. A.thaliana seedling expression data from ASP treatment

ASP induces the expression of early auxin response genes by activates auxin response, and affects responses to other signals associated with the auxin signaling pathway. We used microarrays to detail the effect of ASP on Arabidopsis global gene expression, and identified distinct classes of up-regulated or down genes during this process Overall design: Seedlings were selected at 1/2MS medium grown with or without ASP for RNA extraction and hybridization on Affymetrix microarrays. ASP is a piperazine compound: 1-[(4-bromophenoxy) acetyl]-4-[(4-fluorophenyl) sulfonyl] piperazine.

ASP通过激活生长素应答通路，诱导早期生长素应答基因的表达，并影响与生长素信号通路相关的其他信号应答。我们采用基因芯片（microarrays）技术详细解析了ASP对拟南芥全基因组基因表达的调控效应，并在此过程中鉴定出不同类别的上调及下调基因。
实验设计：将在添加或不添加ASP的1/2MS培养基中培养的幼苗取材，用于RNA提取及Affymetrix基因芯片杂交实验。ASP为哌嗪类化合物，化学名称为1-[(4-溴苯氧基)乙酰基]-4-[(4-氟苯基)磺酰基]哌嗪。