Expression data from Tbx1 knockout (KO) palatal shlelves

Deletion of Tbx1, a member of the T-box transcription factor gene family, results in abnormal epithelial fusion between the palatal shelves and the mandible, which induces cleft palate by inhibiting elevation of the palatal shelves. We used microarrays to determine the downstream genes of Tbx1 during palatogenesis and identified distinct classes of dysregulated genes. Wild-type and Tbx1KO head or palatal shelves were collected at Embryonic day (E) 12.5 or 13.5 for RNA extraction and hybridization on Affymetrix Mouse Gene 2.0ST Array.

T-box转录因子基因家族（T-box transcription factor gene family）成员Tbx1的基因缺失，会引发腭突与下颌骨之间的异常上皮融合，该异常融合通过抑制腭突抬升过程诱发腭裂。本研究采用微阵列（microarrays）技术，在腭发育（palatogenesis）进程中解析Tbx1的下游靶基因，并鉴定出多类表达失调的基因。我们于胚胎第12.5天（E12.5）与胚胎第13.5天（E13.5）采集野生型（Wild-type）及Tbx1敲除（Tbx1KO）小鼠的头部组织或腭突，提取RNA后在Affymetrix Mouse Gene 2.0ST Array芯片上完成杂交实验。