Raw Data - Hypoxic Preconditioning Promotes Survivals of Human Adipocyte Mesenchymal Stem Cell via Expression of Prosurvival and Proangiogenic Biomarkers

Hypoxic preconditioning of h-AMSC culture has proven to increase the expression of VEGF, SCF, OCT-4, and BCL2 and HSP27. This study demonstrated and explained the existence of a new mechanism of increased h-AMSC survival in cultures with hypoxic preconditioning (O2 1%) via VEGF, SCF, OCT-4, BCL2, and HSP 27.

已有研究证实，对人羊膜间充质干细胞（human Amniotic Mesenchymal Stem Cells, h-AMSC）培养体系进行缺氧预处理，可上调血管内皮生长因子（Vascular Endothelial Growth Factor, VEGF）、干细胞因子（Stem Cell Factor, SCF）、八聚体结合转录因子4（Octamer-binding Transcription Factor 4, OCT-4）、B细胞淋巴瘤2（B-cell Lymphoma 2, BCL2）及热休克蛋白27（Heat Shock Protein 27, HSP27）的表达水平。本研究证实并阐释了一种全新的作用机制：在1%氧浓度的缺氧预处理培养条件下，通过调控上述VEGF、SCF、OCT-4、BCL2及HSP27的表达，可提升人羊膜间充质干细胞的存活能力。