Assessing the diversity and spatial distribution of nematodes in the Store Mosse National Park (Sweden) using metabarcoding

Nematode taxa of the Store Mosse National Park in the south of Sweden were surveyed using DNA metabarcoding. Samples were collected from a range of media across all five vegetation types the park spans. A total of 50 samples consisting of soil, litter, lichens, sphagnum, roots, wood, moss, fungus and anthill materials were analysed. Nematodes were characterised using a ~350 bp region of their 18S ribosomal RNA gene. The analysis identified 47 families, 76 genera (25 new to Swedish fauna) and 60 species (32 new to Swedish fauna).

本研究采用DNA宏条形码（DNA metabarcoding）技术，对瑞典南部Store Mosse国家公园内的线虫类群进行了调查。研究人员从该公园涵盖的全部5种植被类型中，采集了多种基质的样品；本次分析共纳入50份样品，涵盖土壤、枯落物、地衣、泥炭藓（sphagnum）、根系、木材、苔藓、真菌及蚁冢材料。本研究以线虫18S核糖体RNA（18S ribosomal RNA）基因约350 bp的区段作为分子标记，对样品中的线虫进行分类鉴定，最终共鉴定出47个科、76个属（其中25个属为瑞典动物区系新记录）以及60个种（其中32个种为瑞典动物区系新记录）。