Figure S1 - Enforced Expression of the Transcriptional Coactivator OBF1 Impairs B Cell Differentiation at the Earliest Stage of Development

(A) FACS analysis of the spleen and peritoneal cavity. B cells were labeled with an anti-CD19-PE antibody. The B2 cells were stained with anti-CD23-FITC. The B1a cells were stained with anti-CD5-FITC antibody or with anti-CD11b-FITC antibody. (B) B1, B1a, B1b and B2 B cell populations in the spleen and peritoneal cavity. The B2 and MZB/B1 B cells are CD19+CD23+ and CD19+CD23- populations respectively. The B1a and B1b B cells are CD19+CD5+ and CD19+CD23-CD5- populations respectively. The histograms represent the mean��SD of three individual mice per genotype. (2.58 MB EPS)

(A) 脾脏与腹腔的荧光激活细胞分选术（Fluorescence Activated Cell Sorting，FACS）分析：B细胞经抗CD19-PE抗体标记；B2细胞采用抗CD23-FITC染色；B1a细胞分别使用抗CD5-FITC抗体或抗CD11b-FITC抗体完成染色。(B) 脾脏及腹腔内的B1、B1a、B1b与B2 B细胞亚群。其中B2细胞与MZB/B1 B细胞分别对应CD19+CD23+与CD19+CD23-细胞群体；B1a与B1b B细胞则分别对应CD19+CD5+与CD19+CD23-CD5-细胞群体。所有直方图均展示了每种基因型下3只独立小鼠的平均值±标准差。(2.58 MB EPS)