A comprehensive map of alternative polyadenylation in African American and European American lung cancer patients

We report the application of a novel method and analysis pipeline that sequences 3'end -enriched RNA to comprehensively map the APA landscape in lung cancer. The goals of this study are to identify the functional role of APA in determining transcriptomic heterogeneity in lung cancer and population differences in APA. Overall design: Ninety-eight patients with histologically confirmed non-small cell lung cancer were selected from the National Cancer Institute-Maryland (NCI-MD) lung cancer study. The study includes 98 human tumor tissues and 98 adjacent non-involved tissues (n=196 total). AA = African American EA = European American

本研究报道了一种新型方法与分析流程的应用，该方法通过对3′端富集RNA进行测序，以全面绘制肺癌的可变多聚腺苷酸化（Alternative Polyadenylation，APA）图谱。本研究的目标为明确APA在决定肺癌转录组异质性中的功能作用，以及APA的人群差异。实验设计：从美国国家癌症研究所-马里兰州（National Cancer Institute-Maryland，NCI-MD）肺癌研究队列中筛选出98例经组织学确认的非小细胞肺癌患者。本研究共纳入98份人体肿瘤组织与98份癌旁正常组织，总计196份样本。AA即非裔美国人（African American），EA即欧裔美国人（European American）