NOTCH-YAP1/TEAD-DNMT1 axis drives hepatocyte reprogramming into intrahepatic cholangiocarcinoma [RNA-Seq]. NOTCH-YAP1/TEAD-DNMT1 axis drives hepatocyte reprogramming into intrahepatic cholangiocarcinoma [RNA-Seq]

We reported the transcritpome in the healthy control liver of wild-type mice, liver bearing myrAKT-NICD driven ICC and livers bearing myrAKT-YAP driven Sox9 deleted HCC from liver-specific Sox9 knockout mice Overall design: Liver samples were taken at 5 weeks post hydrodynamic tailvein injection of myrAKT-NICD or myrAKT-YAP expression plasmid

本研究报道了三类样本的转录组（transcriptome）特征：野生型小鼠的健康对照肝脏、携带myrAKT-NICD驱动的肝内胆管癌（ICC）的肝脏，以及来自肝特异性Sox9敲除小鼠、携带myrAKT-YAP驱动的Sox9缺失肝细胞癌（HCC）的肝脏。整体实验设计：对小鼠实施流体动力尾静脉注射myrAKT-NICD或myrAKT-YAP表达质粒，于注射后5周采集肝脏样本。