Single cell RNA-seq of Gif lineage cells in homeostasis and upon injury

The purpose of this experiment is to assess and characterize injury response of the gastric chief cells in trancriptomic level. Gif lineage cells (TdTom- GFP+) from Gif-rtTA; TetO-Cre; Rosa-mTmG mice line after Dox administration were isolated from the mouse gastric corpus without injury or at 2d after DMP777-mediated acute injury.本实验旨在从转录组水平评估并表征胃主细胞（gastric chief cells）的损伤应答特征。经多西环素（Dox）给药后，从Gif-rtTA; TetO-Cre; Rosa-mTmG小鼠品系中分离得到Gif谱系细胞（Gif lineage cells，TdTom⁻ GFP⁺），这些细胞分别取自未受损伤的小鼠胃体部，以及经DMP777介导的急性损伤后2天的小鼠胃体部。