TAT.ARC protein transduction or RNA interference with ARC impact on survival of primary cortical neurons subjected to 150 min oxygen-glucose deprivation (OGD): Lactate dehydrogenase activity was measured as a marker of cell membrane integrity loss and neuronal cell death 24 h afterwards.

Primary neuronal cultures were transduced with lentiviral particles for knockdown of apoptosis repressor with CARD domain (ARC) or non-targeting control or treated with TAT proteins for ARC or beta Galaktosidase before 150 min of oxygen glucose deprivation (OGD). Cell death was monitored by the release of lactate dehydrogenase (LDH) in the supernatant medium.

将原代神经元培养物经慢病毒颗粒转导，以敲低含CARD结构域的凋亡抑制因子（apoptosis repressor with CARD domain，ARC），或采用非靶向对照进行转导；或是在氧糖剥夺（oxygen glucose deprivation，OGD）处理150分钟前，使用靶向ARC的TAT蛋白或β-半乳糖苷酶（beta Galaktosidase）处理细胞。随后通过检测细胞培养基上清中乳酸脱氢酶（lactate dehydrogenase，LDH）的释放量，监测细胞死亡情况。