Epigenome analysis of hiPSC-based tumour models I. Epigenome analysis of hiPSC-based tumour models I

Generation of in vitro models of cancer stem cells for in vivo xenograft models. hiPSC differentiation into a desired tissue allows studying physiologic processes in human-relevantcell systems. By introducing disease-specific mutations, these cells allow studying genetic origins of diseases in a tissue-specific manner. Overall design: Bisulphite converted DNA from the samples were hybridised to the Illumina Infinium MethylationEPIC Kit array

用于体内异种移植模型的癌症干细胞体外模型构建。人类诱导多能干细胞（human induced pluripotent stem cells, hiPSC）定向分化为目标组织后，可在与人类生理相关的细胞系统中开展生理过程研究。通过引入疾病特异性突变，此类细胞能够以组织特异性的方式助力疾病遗传起源的探究。实验整体设计：将样本经亚硫酸氢盐转化得到的DNA，与Illumina Infinium MethylationEPIC Kit阵列进行杂交。