Untargeted Metabolomics and Quantitative Label-free Proteomics Analysis of Whole Milk Protein from Chinese Native Buffaloes

This study aimed to analyze metabolite abundances and proteome differences between Binglangjiang buffalo milk (BBM) and Dehong buffalo milk (DBM). Untargeted ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), label-free quantitative proteomics approaches, and bioinformatics analyses including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI) were performed.

本研究旨在分析槟榔江水牛乳（Binglangjiang buffalo milk，BBM）与德宏水牛乳（Dehong buffalo milk，DBM）之间的代谢物丰度及蛋白质组差异。本研究采用非靶向超高效液相色谱-串联质谱（UPLC-MS/MS）、无标记定量蛋白质组学方法，并开展包括基因本体（Gene Ontology，GO）、京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）以及蛋白质相互作用（protein-protein interaction，PPI）在内的生物信息学分析。