Summary of the disruption rates of the abundant genes.

The genes of high abundance (normalized percentage >0 in Fig. 5a) were used for the estimation of disruption rate. The gene number differed among the samples. The lengths of the reads and the ORFs identified therein were averaged. The RRP is the ratio of real average ORF length to predicted ORF length (see Materials and Methods). The disruption rate was estimated on the basis of a simulation in reference to the RRP value. SD, standard deviation.

本研究选取图5a中归一化占比＞0的高丰度基因用于断裂率的估算。不同样本间的基因数量存在差异。我们对测序读段及其所识别的开放阅读框（Open Reading Frames, ORFs）的长度取平均值。RRP为实际平均开放阅读框长度与预测开放阅读框长度的比值（详见材料与方法）。断裂率参照RRP值通过模拟分析进行估算。SD即标准差（standard deviation）。