Effects of miRNA overexpression on keratinocytes in mice

Extracellular vesicle(EV) secretion and capture is an important method of communication between cells. Melanocytes and their surrounding keratinocytes form epidermal melanin units in the thin, outermost layer of the skin to protect the skin from ultraviolet radiation damage. It is important to understand the mechanisms by which changes in melanocyte EV signal transduction influence pigmentation. EVs from melanocytes were extracted and used to investigate changes in the miRNA profile of EVs, and the effect of exosomal miR-28-3p on keratinocyte with transcriptomics was analyzed by RNA-seq analysis.Our results indicate that the content of miR-28 in EVs may be reduced to induce keratinocytes to prevent carcinogenesis, resist infection and prevent premature apoptosis. Overall design: mRNA profiles of miR-28-3p overexpression and Control in mice melanocytes

细胞外囊泡（Extracellular vesicle, EV）的分泌与摄取是细胞间通信的重要方式。黑色素细胞与其周围的角质形成细胞在皮肤最外层的薄层结构中形成表皮黑色素单位，以保护皮肤免受紫外线辐射损伤。阐明黑色素细胞EV信号转导的变化如何影响色素沉着的机制，具有重要研究价值。本研究提取了黑色素细胞来源的细胞外囊泡，用以探究其微小RNA（microRNA, miRNA）谱的变化，并通过RNA测序（RNA-seq）结合转录组学分析，解析了外泌体miR-28-3p对角质形成细胞的调控作用。本研究结果显示，降低细胞外囊泡中miR-28的含量，可促使角质形成细胞抵御癌变、抵抗感染并抑制细胞过早凋亡。整体实验设计：小鼠黑色素细胞中miR-28-3p过表达组与对照组的mRNA表达谱。