Supplementary material for manuscript submission at Diversity and distributions

Supplementary data for the manuscript submission at Diversity and distributions entitled: "<i>Gaps and data ambiguities in DNA reference libraries: A limiting factor for molecular-based biodiversity assessments using Arabian Peninsula annelids as a case study</i>"<b>Checklists:</b>Arabian Checklist (CL-MTAP)Mediterranean Checklist (CL-MTMS)<b>BOLD Annelida FASTA COI dataset:</b>DS-MTWA<b>ARMS Metabarcoding ASV obtained FASTA dataset:</b>DS-ARMS<b>Supplementary Tables and Figures:</b><b>Appendix S1 (Table S1)</b><b>:</b> Obtained ASVs and respective number of reads per ARMS and respective taxonomic identification<b>Appendix S2 (Table S2)</b><b>:</b> ARMS metadata with information regarding number of ARMS deployed by region, year and respective coordinates.<b>Appendix S3 (Fig. S3.1)</b><b>:</b> The number and percentages of species (A), genera (B) and families (C) from the Annelida Mediterranean checklist (CL-MTMS) with available DNA barcodes in the BOLD database. (D) Number of available sequences for the most represented families with DNA barcodes in CL-MTMS. (E) Number of barcoded species and species yet to be barcoded for the top10 most represented families in CL-MTMS.<b>Appendix S4 (Fig. S4.1)</b><b>:</b> Neighbour-Joining phylogenetic Tree using Kimura 2-parameters model for DS-ARMS. All 5375 ASVs are individually shown with the respective automatic taxonomic assignment. Discordant clades (based on ASAP methodology) highlighted in red.Public DOIs for specific BOLD related datasets will be available upon paper acceptance.

《多样性与分布》期刊投稿稿件的补充数据，标题为：<i>DNA参考库中的空白与数据模糊性：以阿拉伯半岛环节动物为案例研究的分子生物多样性评估限制因素</i><b>物种清单：</b>阿拉伯半岛物种清单（CL-MTAP）地中海物种清单（CL-MTMS）<b>BOLD环节动物门FASTA格式COI数据集：</b>DS-MTWA<b>ARMS宏条形码获得的扩增子序列变体（ASV）FASTA格式数据集：</b>DS-ARMS<b>补充表格与图表：</b><b>附录S1（表S1）：</b>各ARMS样本获得的扩增子序列变体（ASV）及其对应读数数量与分类鉴定结果<b>附录S3（图S3.1）：</b>地中海环节动物门物种清单（CL-MTMS）中在BOLD数据库有可用DNA条形码的物种（A）、属（B）和科（C）的数量及占比；（D）CL-MTMS中拥有DNA条形码的代表性科的可用序列数量；（E）CL-MTMS中前10个代表性科的已条形码物种与待条形码物种数量<b>附录S4（图S4.1）：</b>基于Kimura双参数模型构建的DS-ARMS数据集邻接（Neighbour-Joining）系统发育树；所有5375个扩增子序列变体（ASV）均单独展示，并附有各自的自动分类学注释；基于ASAP方法识别的不一致分支以红色突出显示。特定BOLD相关数据集的公共DOI将在论文接受后提供。